Journal:

Article Title: Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation

doi:

Figure Lengend Snippet: Nanobacteria cultured under SF conditions and their interaction with cells. (A) Light microscopic micrograph. (B) DNA staining of the same area with the modified Hoechst staining method. (C) DIC images of nanobacteria inside a common apatite shelter. (D) A partly demineralized nanobacterial group (A–D, ×860). (E and F) SEM micrographs of nanobacterial dwellings detached from the culture vessel. (Bars = 1 μm.) (G) IIFS of internalized mineralized nanobacteria (white arrows) in 3T6 cells. (H) DNA staining of the same area with standard Hoechst method. (×540.) (I–L) TEM micrographs of intracellular calcifications in 3T6 cells caused by SF nanobacteria. (Bars: I and K = 2 μm; J = 500 nm; L = 200 nm.)

Article Snippet: 3T6 cells (ATCC CCL 96) were cultured on coverslips as described before ( 5 ).

Techniques: Cell Culture, Staining, Modification

Journal:

Article Title: Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation

doi:

Figure Lengend Snippet: Examples of extra- and intracellular calcification by nanobacteria. TEM micrograph of cultured nanobacteria (bar = 200 nm) from FBS (A) and a bacterium in a kidney stone after demineralization (B; bar = 50 nm). (C) IIFS of the same kidney stone with anti-nanobacteria mAb. (D and E) von Kossa staining results of 3T6 cells exposed to SF nanobacteria for 24 hr. (F) Negative control. (×270.)

Article Snippet: 3T6 cells (ATCC CCL 96) were cultured on coverslips as described before ( 5 ).

Techniques: Cell Culture, Staining, Negative Control