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Image Search Results
Journal: bioRxiv
Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease
doi: 10.1101/2020.04.04.996892
Figure Lengend Snippet: Microglial cells were incubated with three different doses of JN403 (100 nM, 1 μM, and 10 μM) for 24 hours, followed by an assessment of cell viability using the MTT-assay. (A) JN403 treatment alone did not elicit cellular toxicity in microglial cells at concentrations up to 1 μM. non-significant (n.s). (B) Microglial cells were pre-treated with 100 nM or 1 μM of JN403 for 24 hours and additionally coincubated for another 24 hours with 1μM αSyn fragment 61-140 on the next day. αSyn induced cytotoxicity in microglial cells. Neither 100 nM nor 1 μM of JN403 treatment changed the cell viability. Data are presented as the mean ± standard error of the mean. Cell viability was measured for multiple independent experiments. **** p < 0.0001, significantly different from the control group.
Article Snippet: Briefly,
Techniques: Incubation, MTT Assay, Control
Journal: bioRxiv
Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease
doi: 10.1101/2020.04.04.996892
Figure Lengend Snippet: Inflammatory parameters (A) Nitric Oxide (NO) and (B) TNF-α were measured in JN403 pre-treated microglial cells for a baseline. (A) NO, and (B) TNF-α release increased with 1μM αSyn incubation, but both 100 nM and 1 μM of JN403 co-treatment significantly reduced NO and TNF-α release, * p < 0.05, ** p < 0.01.
Article Snippet: Briefly,
Techniques: Incubation
Journal: bioRxiv
Article Title: JN403, an alpha-7-nicotine-acetylcholine-receptor agonist, reduces alpha-synuclein induced inflammatory parameters of in vitro microglia but fails to attenuate the reduction of TH positive nigral neurons in a focal alpha-synuclein overexpression mouse model of Parkinson’s disease
doi: 10.1101/2020.04.04.996892
Figure Lengend Snippet: Iba1+ microglial cells were detected and the density analysis showed no differences between the rAAV groups and treatment groups. (A) Representative photomicrographs showing Iba1+ signals per group, TH (green) and Iba1 (magenta). No activated forms of microglia were found. Scale bar 200 μm. (B) The ratio of Iba1+ density of the injected side compared to the non-injected side showed no significant differences between the rAAVs and treatment, n=4, non-significant (n.s).
Article Snippet: Briefly,
Techniques: Injection